rabbit polyclonal h22 cdk4 (Cell Signaling Technology Inc)
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Rabbit Polyclonal H22 Cdk4, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 93 article reviews
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1) Product Images from "Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27"
Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27
Journal: Cell Cycle
doi: 10.1080/15384101.2021.1984663
Figure Legend Snippet: Screening of anti-phosphoT172 CDK4 mAbs using immobilized cyclin D3/CDK4 proteic fusions.
Techniques Used:
Figure Legend Snippet: Sera evaluation by CDK4 peptide ELISA.
Techniques Used: Peptide ELISA
Figure Legend Snippet: Evaluation of CDK4 T172-phosphospecificity of immune sera.
Techniques Used:
Figure Legend Snippet: CDK4 T172-phosphospecificity of cloned mAbs.
Techniques Used: Clone Assay
Figure Legend Snippet: T172-phosphospecific CDK4 sandwich ELISA.
Techniques Used: Sandwich ELISA
Figure Legend Snippet: S130-phosphorylated p21 is enriched in phospho-CDK4 immunoprecipitation.
Techniques Used: Immunoprecipitation
Figure Legend Snippet: S10-phosphorylated p27 is enriched in phospho-CDK4 immunoprecipitation.
Techniques Used: Immunoprecipitation
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Peptide ELISA:Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27 Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E, Clone Assay:Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27 Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E, Sandwich ELISA:Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27 Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E, Immunoprecipitation:Article Title: Monoclonal antibodies to activated CDK4: use to investigate normal and cancerous cell cycle regulation and involvement of phosphorylations of p21 and p27 Article Snippet: Equal amounts of whole cell extract protein were separated according to molecular mass and immunodetected using the following antibodies: monoclonal mouse antibodies against cyclin D3 (DCS-22) (Neomarkers/ThermoFisher); cyclin D1 (DCS-6), p16 (DCS-50), CDK4 (DCS-31) (Santa Cruz Biotechnology); p21, p27, CDK4 antibodies (respectively 12D1, D69C12, D9G3E, |